Background: Transfusion reactions are adverse events associated with the transfusion of whole blood or one of its components. There is no institutional or national data in Ethiopia regarding the incidence of ATR & their risk factors. The aim of this study is to measure the incidence of acute transfusion reactions (ATR), frequency & time of occurrence of each ATR & evaluate their association with risk factors there by contributing to the institutional & national hemovigilance system.

Objective: The main objective of the study is to measure the incidence of ATR & determine their association with different clinical variables. 

Methods: Prospective cross sectional study design is used to study in patient & outpatient adult transfusion recipients at the department of internal medicine, data was collected with a structured check list, analyzed with IBM SPSS version 24 software.

Results: A total of 210 study participants with the age of 13 & above were included in the study from September 1, 2022 – November 30, 2022. 50.5 % of study participants are male & 49.5% are females, the most common age group are between the age of 18 -40, the commonest blood group was O positive. Acute myelogenous leukemia is the commonest underlying diagnosis of the study participants; PRBC is transfused for the majority of patients given for 74.8% of cases. ATR incidence is found to be 10%, ATR was reported in 19.6% of platelet transfused patients as compared to 7% of PRBC, and none of FFP transfused patients develop ATR. FNHTR & urticaria are the commonest ATR observed. Significant association is seen between ATR & female gender, autoimmune disease & ABO incompatible platelet transfusion. 

Conclusion: The incidences of acute transfusion reactions is higher than reported from previous studies indicating the need to improve institutional transfusion services particularly in high-risk patients.

A blood transfusion is an acute intervention, implemented to solve life and health-threatening conditions on a short-term basis by infusion of donated whole blood or blood components like RBCs, platelets, white cells & the different coagulation proteins.[ 1] Blood transfusion remains a common practice in the management of life threatening clinical situations like trauma, surgical blood loss, severe anemia & different bleeding disorders to replace missing clotting factors and immune system elements. [2,3] Although Blood transfusion is a back bone for the management of a variety of medical & surgical patients, it is not without risks & complications.

Approximately 20%of all transfusions may lead to some type of adverse reactions. Complications associated with blood transfusion therapy may be classified based on time of onset as acute and late transfusion reactions or based on etiology as immunological and non-immunological.

Early onset transfusion reactions are usually acute reactions that occur during transfusion or anytime within 24 hours following transfusion of the blood or blood components, while late reactions occur from 24 hours to 2 weeks following the transfusion. [4]

ATRs include acute hemolytic transfusion reaction (AHTR), allergic reactions, febrile non-hemolytic transfusion reaction (FNHTR), transfusion associated circulatory overload (TACO), and transfusion related acute lung injury (TRALI) and anaphylactic reactions. [5] 

The type and severity of transfusion reactions vary with the transfused blood product, the clinical condition of the recipient, past medical history and age of the recipient. [6]

Despite the increasing public awareness on the risks and complications of blood transfusion that has resulted in a more stringent approach to donation, testing, and preparation of blood and its components, blood transfusion still is not without complications.

Thus, it is important for health professionals to monitor patients during and after transfusion & it is essential to establish a system for monitoring, recording and reporting adverse reactions caused by blood transfusion in each hospital, thereby contributing to the national hemovigilance System. [7]

Reports of blood component transfusion reactions are variable across the globe depending on the quality of the blood transfusion service at the center. The frequency of the complications is however inversely related with the care exercised in the preparation for and supervision of the transfusion [8]. In the developing countries blood transfusion services are fragmented, nonuniform, with different levels of care depending on the institution which will increase risk of ATRs. Reported incidences of ATR differ significantly while incidence of 0.2% and 0.34% are reported in Europe and South America, the incidence of acute immune-mediated transfusion reactions is reported to be 11.8% in North East Nigeria [9].

Learning from transfusion complications can drive the introduction of measures to enhance the quality, safety, efficacy and cost-effectiveness of blood and blood products as well as of the donation and transfusion processes [10]. 

The risk factors for common ATRs are reported only in few research papers & reviews performed before.

In Ethiopia the incidence of ATRs & their associated risk factors is not known & there is no organized institutional hemovigiliance system which record & report ATRs to the national blood bank. 

The aim of this study is to measure the incidence of patients who developed ATRs, frequency & time of occurrence of each ATR & evaluate their association with risk factors from patients transfused with blood components at medical wards, ICU & outpatient hematology unit transfusion department of Tikuranbessa specialized hospital during the study period there by contributing to the safe blood transfusion practice & improving the blood transfusion surveillance system in the hospital.

2.1 Study design

A Cross-sectional prospective study was conducted from patients who was transfused with Blood components at Medical wards, ICU & outpatient transfusion department of Tikuranbessa specialized hospital during the study period.

2.2 Study period

The study was conducted from September 1, 2022 to November 30, 2022

2.3 Study area

The study was conducted at TASH which is located in Addis Ababa, the capital city of Ethiopia. It is the biggest referral hospital in the country with 700 inpatient beds and providing service to an estimated 500,000 patients annually. It also serves as a teaching hospital for undergraduate & post graduate medical & other health science students under the administration of adissababa university college of health sciences.

2.4 Study population 

All Patients who were transfused with different Blood components at Medical wards, ICU & outpatient transfusion department of TASH during the study period.

2.4.1 Inclusion criteria 

 - Age ≥13 years

 - Patients who were transfused with blood products at Internal medicine department wards & medical ICU 

 - Patients who received blood component transfusion at outpatient hematology unit transfusion department.

2.4.2 Exclusion criteria

- Patients Age <13>

- Patients who received transfusion at hospital units other than Internal medicine

- Wards, medical ICU & outpatient hematology unit

3.1 Socio demographic characteristics of the study participants

From September 1 to November 30, 2022, a total of 210 patients who were transfused with different blood component during the study period were included in the study. 106 (50.5 %) of patients are males & 104 (49.5%) are females. The study patients were categorized with the age group & the most common age group included in the study were between age group 18- 40 accounting for 92 (43.8%) of patients.

Figure 1: Age & sex distribution of study participants

3.2 Blood Group & diagnosis of study participants

The most common blood group identified among the study participants was O blood group which account for 40 % of the study population, others include blood group B (27.2%), A (26.2%) & AB (6.7%) of patients. The RH status of the study also studied & showed 198 (94.3%) were RH positive & 12 (5.7%) were RH negative. Considering both the blood group & Rh status of the patients O+ve is the commonest blood group seen in 79 (37.6%) of patients.

The three most common medical problems identified among the study participants were Acute myelogenous leukemia, Aplastic anemia & Chronic myelogenous leukemia which are observed in 62 (29.5%),32 (15.2%) & 24 (11.4%) patients respectively.

Figure 2. Blood group of study participants

3.3 Type of blood components transfused

Blood components transfused for the study population were Packed RBC, Platelets & FFP which were given for 157 (74.8%), 51(24.3%), 2 (1 %) patients respectively.

3.4 Proportion of Acute Transfusion reaction 

From the total of 210 transfused patients with different blood component products 21 (10%) patients develop acute transfusion reaction during the study period; 11 of which are seen among patients transfused with PRBC,10 patients develop ATR after platelet transfusion. Based on patients subjective report of ATR 32 (15.2%) of patients developed at least one episode of ATR previously.

3.5. Type of ATR

FNHTR & urticaria are the only acute complications of transfusion identified during the study period which were seen in 12 (5.7%) & 9 (4.3 %) patients respectively.

When ATR were analyzed according to blood components, ATR was seen in 11 (7%) patients transfused with packed RBC &10 (19.6%) patients transfused with platelets, no patients transfused with FFP developed ATR.

3.6 Time of occurrence of ATR 

The time of occurrence of ATR was variable among study subjects. The majority of patients (52.4%) with ATR develop with in a period of one to six hours.

Table 1 - Time of occurrence of ATR

3.7 Association of ATR with different clinical variables 

3.7.1 ATR & Gender 

From patients who developed ATR 15 (71.6%) were females & 6(28.6%) were males. There is significant association between the patients’ gender & the development of ATR with two-sided significance P value of 0.04 by Pearson chi-square & Fischer’s exact test.

3.7.2 ATR & Age

ATR developed most commonly in age group 18 - 40 years accounting for 47.6 % of ATR cases but there was no significant association between age group differences in the development of ATR with Pearson chi-square test P value of 0.21.

3.7.2 ATR & Blood group

There was no significant association between the blood group of the study subjects & the development of ATR with Pearson chi-square test p value of 0.09.

3.7.3 ATR & blood component transfused

Among 157 patients transfused with Packed RBC only 7% of patients developed ATR but 19.6 % of patients among 51 platelet transfused patients developed ATR. Large number of patients who are transfused with platelets developed ATR than those transfused with PRBC & FFP & there is a significant association between type of blood component transfused & the development of ATR with a Pearson chi-square P value of 0.03.

3.7.4 ATR & Previous history of transfusion

Out of the 32 patients with previous history of transfusion 8 patients developed ATR & there is no significant association observed between current ATR & previous history of transfusion with 2-sided significance level of 0.06 (P >0.05) by Pearson chi-square test.

P = 0.06 (Fisher exact) Chi-square = 9.438 df = 1

Table 2 – Distribution of ATR by previous transfusion

3.7.5 ATR & autoimmune disease

11 patients (5.7 %) of the total study population were found to have autoimmune diseases. From those who developed ATR 4 (19%) patients has autoimmune disease documented & the association between ATR & the presence of autoimmune disease is observed with Pearson chi square P value of 0.011.

3.7.6 ATR & ABO incompatibility

ABO incompatible transfusion was seen in 6 (2.9%) of the study patients & all were observed during platelet transfusion. ABO mismatch transfusion is seen in 60 % of platelet-transfused patients who developed ATR.

 Significant association observed between the presence of ABO incompatibility & ATR with Chi square lambda correlational analysis P value of 0.013.

A total of 210 blood recipients participated in this study, out of which 106 (50.5 %) of patients are males & 104 (49.5%) are females with M; F ratio of 1.1:1 & the commonest age group reported to receive blood component transfusion during the study period are the age group between 18- 40. 

The commonest diagnosis reported among transfusion recipients is acute myelogenous leukemia which accounts for 29.5% of the study subjects which is different from the study reported in Nigeria by Baffa A. et al., which reported the commonest diagnosis among the blood recipients was HIV/AIDS accounting for 16.1

The incidence of ATR is 10 % of the studied population which is higher than the reports from other studies. FNHTR is the commonest ATR seen in 5.7 % & urticaria was seen in 4.3 % of the study subjects. ATR is seen commonly in platelet transfused patients as compared to PRBC or FFP recipients. Significant association is seen between ATR & female gender, the presence of auto immune disease & ABO incompatible platelet transfusion unlike other studies in which significant association of ATR was seen with previous transfusion history, previous abortion history & baseline leukopenia & storage time of the blood product.

The commonest time of occurrence of ATR is the first six hours after initiation of the transfusion.

It is recommended from this study that Blood transfusion is not without risks, transfused patients need due attention & close follow-up particularly during the first six hours of transfusion. Female patients especially those with autoimmune disease need special attention due to the strong association with the development of ATR. 

Platelet transfused patients need to be followed carefully for the development of ATR & ABO mismatched platelet transfusion need to be avoided as much as possible by advocating single donor platelet transfusion practice.

This study is one of the few studies performed on transfusion medicine & the 2nd of its kind reporting the incidence of ATR & associated factors in the country so far. It is a prospective crossectional study which tries to assess the incidence of ATR, associated factors & their occurrence time in a setup where there is no strong transfusion reaction reporting system which will help to contribute data to the development of institutional & national hemovigilance system. 

Small sample size, short study period & the limited group of patients studied are the limitations of the study which will make the generalization of the results to the wider transfusion recipient population difficult; therefore it is recommended to study wider population over a longer period of observation in the future so that the incidence of ATRs including those that are not identified in our study will be determined.