Mangroves are woody trees or shrubs salt marsh halophytes that encompass over 75% of the tropical coastline on the planet. Mangroves are characterized by their sophisticated morphological and physiological adaptations as well as their rich synthesis of biologically active secondary metabolites. They also contribute in decreasing CO2 levels in the atmosphere through photosynthesis. On the other hand, agarwood, is an aromatic perfumed precious heartwood of diseased timber of Aquilaria and Gyrinops trees that is used centuries ago till now as incense in religious ceremonies, raw material in the perfume industry and as a medicine. Agarwood formation is usually induced by wounding trunks and branches of Aquilaria trees. Production of natural agarwood resin is inefficient and don’t meet global demand, so different methods have been applied to stimulate biological agarwood induction, and using endophytic fungi is one of the methods. Hence, this review highlights the importance of endophytic fungi in mangroves and agarwood, their existence and some of their important biological activities.

Mangroves are salt marsh halophytes that are either woody trees or shrubs. 121 nations are home to the mangrove flora. The Mangrove ecosystem refers to the particular areas where mangrove plants flourish. From 6500 B.C., mangrove forestshave been valued for their long-lasting benefits, which have been proven by archaeological evidence. Mangroves are salt-tolerant plant species that grow in estuarine ecosystems and river deltas in tropical and subtropical areas along intertidal zones of rivers and seas in the shape of wide patches or narrow strips. With greater freedom, mangrove species are rapidly dwindling in number [1]. Mangrove forests are found everywhere along the coasts of third-world countries, covering an area of 14 million square miles worldwide. Mangroves encompass over 75% of the tropical coastlineon the planet. Together, the western Pacific and Indian Oceans make up 20% of the world's total area of mangroves [2].

Because the mangrove ecosystem is always under stress, which results in the creation of certainsubstances for their survival, mangrove forests are significant productive tropical ecosystems with high biodiversity [3–4]. Because of the ongoing evolutionary fight for survival, it has been previously suggested that biological diversity would entail chemical diversity [5]. As a result, in addition to their sophisticated morphological and physiological adaptations, the synthesis of bioactive secondary metabolites may be crucial in the ongoing fight between mangroves and other plants, animals, and microorganisms for the scarce resources in their habitat. In fact, despite the fact that comprehensive study on mangrove metabolites has only just emerged, the ability of mangroves to produce a wide variety of bioactive substances is reflected in a number of articles which explain the significant chemical diversity of their metabolites [6-7].

The Rhizophoraceae, Acanthaceae, Lythraceae, Verbenaceae, Combretaceae, and Arecaceae families of shrubs and trees dominate mangrove forests [8]. According to several of these families, Rhizophora mangle(Rhizophoraceae), Laguncularia

racemose (Combretaceae), and Avicennia schaueriana (Verbenaceae), respectively, mangroves in Brazil are categorized as red, white, or black mangroves. The leaves of Rhizophora mucronata are broadly elliptic to rectangular, and its flowers have yellowish stalks [11–12]. The connection of fungi as endophytes with mangrove roots has been hypothesized to provide protection from unfavourable environmental factors and to enable the latter to successfully compete with saprophytic fungi that destroy senescent roots [13]. Moreover, mangroveforests are regardedas an open interface ecosystem that links highlandterrestrial and coastal estuarine environments, and the endophytic fungi in mangroves are a conglomerate of freshwater, marine, and soil fungi [13–14]. Moreover, endophyte assemblage varies with the host plant'sage, different seasons, and different sections(leaves, twigs, roots) [15].

Nonetheless, Avicennia species made inexpensive and nourishing feed for camels,buffaloes, sheep, and goats. These animals are permitted to graze in mangrove habitats, and camels in particular are occasionally taken to uninhabited islands with dense mangrove cover. In Indonesia, the Pakistani Gulf, and India, this is quite typical [16]. Tolerance to various conditions like salt, oxidative stress, hyperosmotic stress, drought, and nutritional stress, for instance, may be conferredby them [17–19].Several ailments are treated with mangroves in traditional medicine [20]. They were utilized for food, energy, medicine, and leather tanning [21]. According to Kathiresan 2012, mangroveextracts may be able to treat human, animal, and plant infections as well as chronic viral diseases like AIDS [22]. Mangroves are recognized to play a part in removing CO2 from the atmosphere through photosynthesis, which may have lessened the issues with "Greenhouse gases" and global warming, according to Kathiresan and Bingham in 2001 [23].

Fungi

Every ecosystem needs fungi because they play a critical role in activities including nutrient recycling, decomposition, and movement across diverse settings. Only a small portion (about 5%) of the over a million distinct fungus species thought to exist on Earth have been recognized [24]. About 120 fungal species, including 87 ascomycetes, 31 mitosporic fungus, and 2 basidiomycetes, were reported by Hyde in 1990 as colonizing 29 mangrove plants around the world [25]. One of the most prevalent characteristics of mangrove forests is the zonation phenomena.It has been reported that 625 mangroveassociated fungus,comprising 279 Ascomycetes,277Mitosporicfungi, 29 Basidiomycetes, 3 Chytridiomycetes, 2 Myxomycetes, 14 Oomycetes, 9 Thraustochytrids, and 12 Zygomytes, exist. One of the crucialmicrobiological elements of plants is fungi. Along with other microorganisms, it forms a crucial component of the ecosystem [28-29]. Manglicolous fungi are mangrove-specific fungus. They are crucial for the cycling of nutrients and can produce all the enzymes needed to break down lignin, cellulose, and other plant materials [30–31]. The variety of fungus species and their seasonalappearance on mangrove seedlings are found on mangroves from all over the world [32]. In the mangrove ecosystem, fungi can be found as saprophytes, symbiotic partners, or parasites. These fungi are divided into two classes: the lower class, which includes Oomycetes and thraustochytrids, and the upper class, which includes ascomycetes and basidiomycetes. Secondary metabolites produced by fungi have a wide range of structural variations, and they frequently serve as a form of self-defence against other microbes. Mangrovefungus are typicallyfound adorning difficult settings, providing them a rich source of bioactive compounds [33].

Endophytic fungi

A classof fungus knownas endophytic fungiinfiltrate the interior and live tissues of plants without immediately manifesting any detrimental effects[34]. Endophytes are obtained from fungus, bacteria, or internal, symptomless plant tissues. Although it creates no visible signs of disease, it targets the tissues of living plants and causes infections that are fully contained within plant tissues [35]. Many investigations on the fungus living in mangroves along the coastlines of the Indian, Pacific, and Atlantic Oceans have been done since Cribb first identified endophytic fungi isolated from mangrove roots in 1955 [36].

According to phylogenetically determined classifications, endophytic fungi fall under the Ascomycota, Basidiomycota, and Zygomycota groups [37]. Trichoderma ascomycetes were originally discovered in mangrove habitats in Brazil, China, and Indonesia in 1920, and they have just recently been publishedin the literature. Trichoderma spp., which are well-known biocontrol agents against diseases of cultivated plants, also exhibit an increased ability of hazardous chemicalbreakdown in plants, soil, and water [38]. Trichoderma spp. colonizes its hosts quickly, produces a lot of free- living, green spores,

and has fruiting bodiesthat help to characterize this  genus' fungi [39]. Another filamentous fungus with branchedmycelium bodies is Zygomycetes of Rhizopus genus, which was discovered in a mangrove area of Nigeria.It is mostly employed as a source of enzymes for the breakdown of organic contaminants and in conventional food fermentation processes [40–41]. The basidiomycete phylum includes the filamentous fungus Schizophyllum commune, which was isolated from an Indian mangrove forest.Its fruiting bodiesand pale to lightgreyish/brown colonies make it easierto identify. It possesses antiviral and anticancer properties and is utilized in the synthesis of pigments [42].

In various nations throughout the world, the diversity of endophyticfungi has been investigated primarily on the barks, branches, leaves, stems, and roots of mangrove plant species in order to determine their biological activity. It is well recognized that endophytic fungi are a rich source of bioactive substances, including medicines and enzymes. Endophytic fungi represent a new source for getting enzymes with various potentialities because they are a relatively untapped field in microorganism isolation. Over one million endophytic species are found throughout all plants, and endophytic fungi are one of these endophytes that live inside the plants without manifesting any symptoms [43].

Biological activities of mangroves endophytic fungi

Because of their immense capacity to create bioactive substances, endophytes are becoming more and more significant. In mangrove fungi, which have led to the discovery of numerous novel bioactive compounds with significant nutraceutical and medicinal applications, endophytes play a crucial role. Together with other pharmaceuticals, these include antibiotics, anticancer, antidiabetic, antioxidant, antiviral, anti-inflammatory, and immunosuppressive medications. Because of their immense capacity to create bioactive substances, endophytes are becomingmore and more important. The choice of natural sources is one of the most important elements of a successful natural product- based drug development program. Despite the limited amount of research on the chemistry of mangrove endophytes, more than 200 species of endophytic fungi have been isolated and identified from mangrove trees and have already been shown to be a reliable source for structurally diverse and biologically active secondary metabolites [44]. Endophytic fungi were considered to be new sources of active chemicals, biological activity, and biotechnological advancements; yet, their full potential has yet to be fully realized[45]. When compared to otherplant parts, leavestypically exhibit a more diversified fungal endophytes community[46– 47]. Significant biological activities for endophytic fungi that originate from mangrove leaves [48–50].

The most common cultivable endophytic fungi in mangroves have been identified as Alternaria, Aspergillus, Cladosporium, Clolleto-trichum, Fusarium, Paecilamyces, Penicillium, Pestalotiopsis, Phoma, Phomopsis, Phyllosticta, and Trichodema [51]. Several studies were shown on microorganisms associated with R. mucronata, including fungal endophytes. An earlier study in secondary metabolites of endophytic fungi from mangroves visible that the fungi produced antibiotic like Griseofulvin, which is generally found in Penicillium griseofulvum [52] on the plant itself. While various isolates of R. mucronata, including Penicillium, Ampelomyces, and Fusarium, were shown to be effective against E. coli, isolates of R. apiculata resulted in the discovery of new chemicals like acropyrone, bicytosporone D, waol acid, and pestalotiopene [53–54]. Alkaloids, terpenes, coumarins, quinones, anthraquinones, quinones, quinones, peptides, phenolicacids, and lactonesare some of the secondary metabolites of mangrove endophytic fungi. From Pestalotiopsis sp., halotolerant Rhizophora stylosa was isolated, and it has the ability to produce lignin-degrading enzymes. As a result of their existence, Alternaria, Aspergillus, Cladosporium, Colletotrichum, Fusarium, Paecilomyces, Penicillum, Pestalotiopsis, Phoma, Phomopsis, Phyllosticta, and Trichodermahave been identified as the main cultivable mangroveendophytic fungi [55].

Agarwood

Agarwood, a precious natural product (Widely used as incense in religious ceremonies, raw material in the perfume industry and a medicine for treating sedative, analgesic and digestive problems for thousands of years), can be defined as aromatic perfumed dark resinous heartwood of diseased timber of Aquilariaand Gyrinops. Agarwood has been used for thousands of years in several countries as incense, perfumes, and multifunctional pharmaceutical product. The main compounds in agarwood includeSesquiterpenes, 2-(2-phenylethyl)- 4H-chromen-4-one and its derivatives (PeCs), Aromatics, Triterpenes and five other categories [56].

Traditionally, agarwood formation is induced by wounding, holing, nailing, and chopping the trunks and branches of Aquilaria trees. According to the latest hypothesis, agarwoodformation is due to plant  defence [57]. When an Aquilaria tree is wounded, damage signals are induced and transmitted to activate the defence response, which subsequently leads to the production of defensive substances such as sesquiterpenes and phenylethyl chromone derivatives [57]. Natural agarwoodresin production is inefficient and yields are low; thus, it is difficult to meet global demand, so different methods have been applied to stimulate biological agarwood induction, and the use of endophytic fungi is one of the methods [57].

Agarwood endophytic fungi

Endophytic fungi have been isolated from several parts of Aquilaria, and some of them show evidence for inducing agarwood formation. Gong & Guo [58], isolated endophytic fungi from leaf, root and stem tissues of Aquilaria sinensis and found that most abundant endophytic fungus is Mycelia sterilia (14.3%) followed by Fusarium sp. (7.1%), Cladosporium edgeworthrae and Glomerularia sp. (5.7%). Some fungal strains are known for antimicrobial activities, but the role of fungal endophytes in the formation of agarwood was not explored. Mohamed et al. [59], showed that a wounded stem of Aquilaria malaccensis contained Fusariumsp. (46%) and Lasiodiplodia sp. (35%), both of which are mainly responsible for agarwood production followed by Trichoderma sp. (13%).

Biological activities of agarwood endophytic fungi

Cui et al. [60], found that endophytic Fusarium and Phaeoacremonium species are the dominantgenera in the stem of A. sinensis, and Phoma herbarum and Fusarium equiseti show promising antimicrobial activities, while Xylaria mali, Phaeoacremonium rubrigenum and Lasiodiplodia theobromae show high antitumor activity [60].

Twenty-eight fungal endophytes were isolated from agarwood by strict sterile sample preparation and wereclassified into 14 genera and 4 taxonomic classes                  (Sordariomycetes,                Dothideomycetes, Saccharomycetes, and Zygomycetes) based on molecular identification. Of the 28 isolates, 13 (46.4%) showed antimicrobial activityagainst at least one of the test strains by the agar well diffusion method, and 23 isolates(82.1%) displayed antitumor activity against at least one of five cancercell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The diametersof inhibition zones of YNAS07, YNAS14,HNAS04, HNAS05, HNAS08, and HNAS11 were equal to or higher than 14.0 mmagainst                             Staphylococcus                    aureus, Escherichia coli, Bacillu subtilis, B. subtilis, Aspergillus fumigatus, and B. subtilis, respectively. The inhibition rates of YNAS06, YNAS08, and HNAS06 were not less than 60% to 293-T, 293-T, and SKVO3cells, respectively [60]. Cui et al., [60], results that the endophytic fungi associated with agarwood will provide us with not only useful micro-ecological information, but also potential antimicrobial and antitumor agents.

Agarwood is the most expensive non-construction wood product in the world. As a therapeutic agent, agarwood can cure some diseases, but few studies have been carried out on the antagonistic abilities of endophytic fungi associated with agarwood. Agarwood is mainly found in the genus Aquilaria[61]. 47 endophytic strains were selected to check their bioactivities against three bacterial pathogens viz. Erwinia amylovora, Pseudomonas syringae, and Salmonella enterica; and three fungal pathogens viz. Alternaria alternata, Botrytis cinerea, and Penicillium digitatum. The 47 strains selected, 40 strains belong to 18 genera viz. Alternaria, Annulohypoxylon, Aspergillus, Botryosphaeria, Colletotrichum, Corynespora, Curvularia, Daldinia, Diaporthe, Fusarium, Lasiodiplodia, Neofusicoccum, Neopestalotiopsis, Nigrospora, Paracamarosporium, Pseudopithomyces, Trichoderma, Trichosporon and one strain belongs to Xylariaceae had antimicrobial activities. In particular, Lasiodiplodia sp. (YNA-D3) showed the inhibition of all the bacterial and fungal pathogens with a significant inhibition rate. In addition, the strains viz; Curvularia sp. (GDA-3A9), Diaporthe sp. (GDA-2A1), Lasiodiplodia sp. (YNA- D3), Neofusicoccum sp. (YNA-1C3), Nigrospora sp. (GDA-4C1), and Trichodermasp. (YNA-1C1) showed significant antimicrobial activities [61].

Studying the endophytic fungi existing on mangroves and agarwood can contribute in further understanding of their importance and role in protection or induction of these ecosystems. Moreover, the secondary metabolites produced by these endophytic fungi are a rich source that can contribute in increasing potency of some currently used drugsor even the invention of newdrugs. Databases shouldbe made to contain all reported data concerning these fungi, their metabolites and their biological activities in order to facilitate providing researchers with updated information which will save time and effort.