Stop the elimination of NOx is an easy method to stop global warming. Elimination of NOx at developed countries induced global warming. Elimination of NOx reduced the CO2 assimilation and decreased fish production and _Average life of Japanese is longest in the world. Reason of long. life is Japanese etc. fish. Fish containing glucosamine, condroitin, gluuronic acid is good for anti-aging. If we stop NOx elimination with ammonia, and if we stop the N, P elimination of drainage, plankton growth is activated and Fish production will increase heat absorption by CO2 assimilation increase and global warming will stop and people can enjoy long life.

1. Stop the elimination of NOx is an easy method to stop global warming. Elimination of NOx at developed countries induced global warming. Elimination of NOx reduced the CO2 assimilation and decreased fish production of fish. Anti-aging is a dream of people for ten thousand years. Average life of Japanese .is men 80.5(third), women

86.83 (top in the world) I wonder why Japanese live longer than other countries. I believe that Japanese food based on fish is good for long life. Following story exist for long time, never throw away umbilical cord (connecting tubes between placenta of mother and unborn baby for the supply of nutrition), naval string and keep it at poulownia chest of drawers for long time, and when you get incurable sickness, boil it in water for long hours, then drink the solution, then heavy sickness will be cured. I wish to explain the reason why umbilical cord (main component is hyaluronic acid) is so effective. Hyaluronic acid chondroitin, glucosamine are now sold from several companies as health food and several million persons are drinking and enjoying health and long life. Author find the reason why these compounds are effective (ref 1-10)

2. Fish contain glucosamine, hyaluronic acid and chondroitin. Glucosamine, hyaluronic acid are now used as health food by many persons in Japan. About 7 million people are drinking and eating these materials and enjoying health and long life. Author studied how glucosamine, hyaluronic acid condroitin contribute for the hearth and anti-aging (ref 1-10)

3. Discovery of Klotho (Anti-aging gene), and Anti-aging reagent) Prof. Nabeshima Yoichi (graduate from Niigata University and Prof at cell engineering center at Osaka University and then Prof at Kyoto University and now head of top medical center.) found Klotho (anti-aging gene) from rat. (ref 11) The rat having this gene live 30 % longer. Klotho keeps homeostasis of Calcium and keeps health. (ref 11-13) Prof Nabeshima also found disacharide which can bind Klotho and FGF27. The molecular weight of disacharide is 843.28 corresponding C30H10N7O19S. Thr-His-Gln-O-beta D-3sulfo-glucopyranoside. The author organic synthesized 5 disaccharides co-work with Klotho from structure known compounds. And found that the following compound can combine with Klotho. The author named this compound as anti-aging reagent. These reagents are combined compounds of gluchronic acid with glucosamine, gluchronic acid with galakutosamine Sulfo-glucunosyl-(1-3) glueoside Sulfogluglucuronosyl-(1-3) galactoside.

Klotho make disaccharide from glucosamine, hyaruronicacid, condroichin on site cowering with Klotho and contributing for health and anti-aging.(ref 11-13)

1. Relation of glucosamine,hyaruronic acid,condroitin,anti-aging teagent,Klotho,health,and anti-aging

2. Klotho has beta glucosidase activity. (tef11) beta- glucosides have activity to cleave and bind glucuroronosy bond. Therefore, Klotho can make dishcharide from hyrunoic acid and conroitin. Hyrunoic acid is poly(glucronyl(1-3) N-acetyl glucoside and condroitin is poly(glucosyl(1-3)N-acetyl galactoside.

3. Sulphonated hiaruronic acid monomer is anti-aging reagent

4. Hyaruronic acid, and coldoitin are precursors of anti-aging reagent. Hyarunoic acid gives anti-aging reagent glueronyl (1-3) N-acetyl glucosamine

5. Klotho

6. Hyaruronic acid =====> Glucuronosyl (1-3) N-acetyl glucosamine

7. Kondroitin                sulphonic acid          give         anti-aging                reagent     sulphoglucrosyl(N- acetylgactasamine by Klotho

8. Klotho

9. Kondroitin sulphate —> sulpho-glucronosyl (1-3) N-acetylgalactosamine

Depolymerizatio of chitin give N-acetyl glucosaminene Klotho

Chitin ———>            N-acetyl glucosamine Depolymerization of chitosan give glueosamine Depolymerization of Chitosan give glueosamine

Klotho

Chitosan ————>   Glucosamine

Klotho condense gluchronic acid and glucosamine to afford disaccharide

Klotho Gluehronic acid                +              glueosamine ———-> disaccharide Relation of gulucosamine derivative, food and anti-aging

When we look for the glucosamine derivatives from natural products

Hyaluronic acid, condroitin, chondroitin sulphate, chitin, chitosan,keratin sulphate.

Hyaluronic acid was isolated from eyeball of caw in1934 and named as hyluronic acid from Greece word glass Hyaloid.

Hyaluronic acid is contained in the eye and indirectly eating of fish, whole body oohed of fisher eye of fishes, like sardine, sea bream, house mackerel, mackerel pike,ayu gold eye sea bream,lorch,eel, flatfish, shrimp, ikanago, kibinago, okoje,mebaru, shirasu, tsumire(crashed mixture small fish) shark fin,tsukudani of small fish, cow, pig, chicken are recommended. Hyaluronic acid is found in the highest concentrations in the eyes and joints. For the supply of glucosamine glucosamine, shrimp and crab are good food. Vegetable anti-aging food. Sticky and nebaneba and draw thread vegetable food like soybean, yamaimo, satoimo, okura, moroheiya, mozuku, konbu, wakame, mekabu and negi cotain mucin. Mucin is suger protain which N-acetylgalactosamine conbine by sugar linkage with serine or threonine.

Fish is the best food for anti- aging. Fish must be produced much with cheap price.

Then enough hyaluronic acid, chondroitin, glucosamine and Calcium are supplied, and Ca homeostasis is maintained and anti-aging, long life will be obtained

Anti- aging, anti-aging food

The author was born in 1930. April 17. 94.4-year-old Author is testing himself what is the best food to live long with high producing manuscripts (76 manuscripts (ref 1-10, 14-79 in 1985-2024) all single author).

How can I live long. I eat night food at midnight one banana, 20g Iriko (boiled and dried sardine), Hatosabure(Kamakura cooky) and Calpis. Then thinking and half sleeping and wake up and write manuscripts.

To get anti-aging

Fish must be supplied much with reasonable price by the mass production using N, P recycling

Climate must be comfortable by cooling with CO2 assimilation Elimination of NOx at developed countries induced global warming.

At around 1980, 7 Developed countries decided the elimination of NOx by ammonia. 

4NO +4NH3 +O2——-> 4 N2 + 6 H2O

This reaction eliminates nitrogen two times

This decision caused global warming by the decrease the nitrogen concentration of sea and land and inhibited CO2 assimilation, decreased the absorption of CO2, decreased the heat absorption by CO2 assimilation. and started global warming (ref 72-78,) After 1980, DGP of Japan does not increase.by. This reaction decreased plankton production, and decreased fish production. This reaction decreased plant growth 。At nitrogen rich sea.CO2 react with water and make carbohydrate very quickly and make plankton.Nitrogen rich land plant absorb CO2 and water and make carbohydrate and cellulose quickly making tree.

CO2 assimilaten

6 CO2 + 6 H20 ——————-> Carbohydrate           + 6 O2

Sun               |

Animals, Fish <————- Plankton, Plant

Tree, Cellulose

Developed countries are eliminating N and P in wastewater Developed contraries are forced to burn garbage at incinerators equipped with ammonia addition.

These NOx and N, P eliminations caused the lack of N, P. CO2 assimilation decreased and CO2 absorption decreased. Food production decreased. Heat absorption by CO2 assimilation Is lost. And global warming has started. Developer countries should stop NOx elimination by ammonia and close wastewater clean center. Then CO2 assimilation is activated, and global warming will stop, and much food will be produced. And people can live long at moderate temperature and can live long (ref 14-78)

News Paper Ehimeshinbun at Ehime Japan reported (ref 14). drainages purification block the production of Nori (Sea weed). Saijo district was a production district of Nori since Edo

300 years. But Nori produced at this district was brown and not black and had no commercial value. At Honjo district 10 km north of Matsuyama 90 person were producing Nori in 1978, but no one producing Nori in 1983.Fourty years ago, at west side of sea shore of Matsuyama, large amount of sea weed were washed ashore, when strong wind blow, and many cucumber (Namako) were there in sea weed.

At west side, there is water purification center. Nitrogen compounds like urea were changed to nitrogen and clean water like drinking water Is released to sea.

DGP of Japan stopped.after 1980. United Nation say why this happened.

AT 1980 Japan started NOx elimination by ammonia. Japan builled 2200 water clean center to eliminate N, P. At 2006, Japan builed 2000 incinerated equipped with ammonia addition.

Author calculated heat balance of earth.on earth, (ref 42,74,77)

Author indicated that global warming can be stopped by increasing CO2 assimilation by stopping NOx, NP elimination, by absorption of heat.

Heat and CO2 generation 140 billion tons of fossil fuel is burned, and CO2 3.6x1010 t is produced. Moreover, 7.4x1015 kcal is produced.

When we consider the heat produced by animal realization, 7.4x1015 kcal is produced. When we consider the electricity generation capacity of the world is 16,868 tera watt h. Electricity generation by atomic energy is 2,086 tera watt h. Therefore, 7.4x1015x2,986/ 10,868=2.02x1015 kcal evolved by atomic energy. The heat evolved by animals also warms the earth. Humans eat 1,000 kcal of food daily and release 1,000 kcal of heat daily. Assuming the population of the world as 7.6 billion, humans release 1,000x365x76x109=2.8x1016 kcal in one year. Animals other than human beings, caw, birds, whales, and seals are producing heat. We can estimate the same as a human being 2.8x1016 kcal. Therefore, to.4+0.202+2.8+2.8) x1016=13.002x1016 kcal. We must absorb 13.002x1016 kcal by CO2 assimilation.

Heat and CO2 absorption

We must absorb 13.002x1016 kcal by CO2 assimilation. One mole of CO2 (44g) and water (18g) absorbs 114 kcal sun’s heat to carbohydrate and 32 g oxygen. If 51 billion t CO2 assimilation occurs 114x5.1x10 /44=13.16x1016 kcal can be absorbed.

Heat production 13.002x1016 kcal1 is almost the same as heat absorption 13.16x1016 kcal36.0+12.5=48.5 billion tones, CO2 can be fixed. And we can absorb heat.13.16 x1016 kcal. Heat absorption by CO assimilation is essential. 14.4 billion tone NOx can fix 14.4x25=360 billion tone CO2. CO2 assimilation must be promoted by stopping NOx elimination. By stopping of NOx elimination, 14.4 billion tone NOx can fix 14.4x 25= 360 billion tone CO2. The amount of NP in drainage is around 0.5 billion tones. Using this 0.5 billion tone NP, people can fix 0.5 X 25= 12.5 billion tone CO2. By adding 360+ 12.5 = 372 billion CO2 can be fixed. And we can absorb 13.002x1016 kcal. Heat absorption by CO2 assimilation is essential to control the earth’s temperature to maintain the habitable and comfortable temperature for live long. (ref 73-78)

Heat generation 13.002 x1016 kcal is almost the same as heat absorption 13.16 x1016 kcal. GWPR =Heat production/ heat absorption=13.002 x1016 kcal. /13.136x1016 kcal. =1 CO2 assimilation must be promoted by absorption of NOx elimination and by stopping wast water elimination. By stopping NOx elimination １４.4 x25＝360 billion tone CO2. The amount of N, P in Drainage is around 10 billion tones. By using this 10 billion tone N, P we can fix 10x 25= 250 billion tone CO2. By adding 360+ 250 = 610 billion tone CO2 can be fixed. And we can absorb 15x 1016 kcal. And earth can be cooled down and we can live long at comfortable temperatures.

Global warming can be stopped by the promotion of CO2 assimilation by supply of nutrient N and P by following 3 items

Stop the elimination of NOx by ammonia at power station, chemical station and iron station Stop the elimination of N, P at drainage, river

Throw away the rules to eliminate NOx by ammonia, throw away the rule to eliminate N, P in drainage, river, throw away the rule to inhibit bon fire, burning of scrap wood

Anti-aging and long life will be accomplished by eating fish.